diff --git a/BaseTools/Scripts/FormatDosFiles.py b/BaseTools/Scripts/FormatDosFiles.py
index 3150bef3c2..e119334ded 100644
--- a/BaseTools/Scripts/FormatDosFiles.py
+++ b/BaseTools/Scripts/FormatDosFiles.py
@@ -53,6 +53,7 @@ def FormatFilesInDir(DirPath, ExtList, Args):
             FileNames[:] = [f for f in FileNames if f not in Args.Exclude]
             Continue = False
             for Path in Args.Exclude:
+                Path = Path.strip('\\').strip('/')
                 if not os.path.isdir(Path) and not os.path.isfile(Path):
                     Path = os.path.join(ExcludeDir, Path)
                 if os.path.isdir(Path) and Path.endswith(DirPath):